SPLIT-STED Imaging of Nuclear Structures

Imaging cellular structures in super-resolution with SIM, STED and Localisation Microscopy: A practical comparison

Many biological questions require fluorescence microscopy with a resolution beyond the diffraction limit of light. Super-resolution methods such as Structured Illumination Microscopy (SIM), STimulated Emission Depletion (STED) microscopy and Single Molecule Localisation Microscopy (SMLM) enable an increase in image resolution beyond the classical diffraction-limit. Here, we compare the individu...

Live-cell imaging of dendritic spines by STED microscopy.

Time lapse fluorescence imaging has become one of the most important approaches in neurobiological research. In particular, both confocal and two-photon microscopy have been used to study activity-dependent changes in synaptic morphology. However, the diffraction-limited resolution of light microscopy is often inadequate, forcing researchers to complement the live cell imaging strategy by EM. H...

Imaging living synapses at the nanoscale by STED microscopy.

The effect of earth magnetic field on the function of nuclear medicine imaging systems (SPECT)

Background: The nuclear medicine systems are very sensitive to the variation of the magnetic field. Photomultiplier tubes amplify low energy light signal and change it into electrical current. Low magnetic field can produce some variations in uniformity and special resolution of SPECT systems. In this study, we tried to evaluate the effects of magnetic field on the function of nuclear medic...

Sub-Abbe resolution: from STED microscopy to STED lithography

Commonly, in stimulated emission depletion (STED) fluorescence nanoscopy, light of a wavelength located at the red tail of the emission spectrum of the dye is used to shrink the effective fluorophore excitation volume and thus to obtain images with sub diffraction resolution. Here, we demonstrate that continuous wave (CW) STED nanoscopy is feasible using STED wavelengths located at the emission...